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Transtracheal aspiration is a technique for the collection of bronchial secretions for laboratory evaluation and culture. This technique is useful when standard sputum collection has not provided adequate material or determination of the infective agent(s). Specimens collected by this technique are free of contamination from nasal, oral, and pharyngeal secretions. Pecora first described this technique in 1959.1 Several modifications to the original technique have been made.2–5 This technique may be more properly named transcricothyroid membrane aspiration.

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The most superficial portion of the cervical airway begins at the inferior thyroid cartilage and extends inferiorly to the thyroid isthmus (Figure 18-1). The inferior border of the thyroid cartilage is attached to the cricoid cartilage by the cricothyroid ligament. This is formed by a thicker central conus elasticus and laterally by thinner ligaments that are covered by the cricothyroid muscles (Figure 18-1B). The internal surface is covered by the mucous membrane of the larynx. Collectively, this is often referred to as the cricothyroid membrane or cricovocal membrane. The paired cricothyroid arteries cross from lateral to medial to form an arch that anteriorly crosses the upper one-third of the cricothyroid membrane. The pyramidal lobe of the thyroid occasionally extends superiorly to this level.

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FIGURE 18-1
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Anatomy of the airway structures of the neck. A. Topographic anatomy. B. The cartilaginous structures.

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Transtracheal aspiration is indicated for the collection of tracheobronchial secretions for laboratory evaluation. Often, previous attempts to collect standard coughed and expectorated sputum samples have failed to yield adequate samples or reveal the etiology of a pulmonary infection. Patients who do not appear to be responding to the appropriate antibiotic regimen that was indicated by evaluation and culture of sputum samples may benefit from this technique to better determine the pathogen(s). This is particularly true in cases of atypical or mixed flora, as in suspected aspiration pneumonias, where this technique may yield superior culture results when compared to sputum samples.6

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Patients who are unable to cooperate with or tolerate the required positioning should not be selected for this technique.7 Agitated patients requiring sedation that may affect respiratory effort should be avoided. Traumatically injured patients should have the cervical spine cleared for possible injury prior to performing the procedure. Patients with known or suspected blood dyscrasias (abnormal platelet counts, elevated prothrombin or partial thromboplastin times) should not be subjected to this technique due to the increased risk of tracheal hemorrhage. The operator must be able to easily identify the patient’s anatomic landmarks, including the thyroid and cricoid cartilages and the intervening membrane space. Patients with abnormal or distorted anatomy should be excluded. Patients who are endotracheally intubated or have a tracheostomy do not require this procedure.

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  • Sterile gown, gloves, and mask
  • Pillow or padding for shoulders
  • Povidone iodine solution or alternative cleansing solution
  • Sterile gauze ...

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