To evaluate a patient’s stool sample for the presence of fecal leukocytes.
Freshly collected liquid stool specimen, glass microscope slide, coverslip, and methylene blue.
Place a drop of liquid stool onto the glass slide.
Add two drops of methylene blue to the stool specimen.
Overlay with a coverslip.
Place the edge of a piece of filter paper adjacent to the coverslip to absorb any excess methylene blue.
Examine using ×10 objective to scan specimen and ×40 and ×100 to identify specific leukocytes.
Fecal Leukocytes. Multiple white cells in the stool specimen from a patient with bacterial diarrhea. (Photo contributor: Herbert L. DuPont, MD.)