Skip to Main Content

We have a new app!

Take the Access library with you wherever you go—easy access to books, videos, images, podcasts, personalized features, and more.

Download the Access App here: iOS and Android


To evaluate for the presence of ring trophozoites.


Air-dried blood smear, Coplin jar of Wright stain, slide rack, pH 7.2 buffer, and blotting paper.


  1. Place a drop of blood on the middle of a slide.

  2. Hold another slide evenly on top of the slide at a 45-degree angle and drag the slide over the drop of blood to the opposite edge to spread the blood evenly.

  3. Allow the blood to dry for 5 to 10 minutes.

  4. Stain air-dried smears in a closed Coplin jar of Wright stain for 5 minutes.

  5. Place the slide on a rack.

  6. Rinse and treat with pH 7.2 buffer primed with 1 mL Wright stain per 400 mL for 3 minutes.

  7. Rinse in pH 7.2 buffer for 20 seconds.

  8. Blot dry and mount on microscope at ×100 (oil immersion).

FIGURE 25.26

Plasmodium falciparum Thin Film. Ring forms (trophozoites) of P falciparum are seen on the Wright stain thin film in a patient with intermittent fever who had recently traveled to Africa. (Photo contributor: James P. Elrod, MD, PhD.)

Pop-up div Successfully Displayed

This div only appears when the trigger link is hovered over. Otherwise it is hidden from view.